Resources

Breeding  |  Mutants  |  Genetics  |  Commercial Services  |  Pathology  |  Groups  |  Books  |  Courses  |  Cores Elsewhere
Mouse as a Model System and How to Breed Mice

• The Mouse as a Model System, information on the evolution, genetics, reproduction and manipution of the mouse.
• Managing A Mouse Colony, information on how to work with mice.
• Targeting Vector Design
• Introduction to Mouse Reproduction and the Postnatal Period from Lee Silver's Mouse Genetics
• UC Irvine University Transgenic Core has an excellent guide to mouse breeding and reproduction
• University of Michigan Transgenic Core guide to breeding transgenic and knockout mice
• Jax Mouse Breeding Strategies Manual (.pdf) (with many plugs for services from The Jackson Laboratory)
• Recommended primers for common transgene components from the Washington University Transgenic Core.
• Reliene & Schiestl (2006) Differences in animal housing facilities and diet may affect study outcomes-a plea for inclusion of such information in publications. DNA Repair 5(6):651-3 (.pdf)

Mutant Repositories:

Mutant Mice, Cryopreserved Embryos and ES cells
• International Mouse Strain Resource. A database with links to repositories of mutant mice, cryospreserved embryos and gametes and mutant ES cells.
• Mouse Genome Informatics at The Jackson Laboratory has an annotated listing of all mutant mice, with links to sources for the mice. Search for your gene by name, and follow the links to the mutant alleles through the phenotypes links. Also links to the IMSR
• Jax Induced Mutant Resource has an extensive collection of targeted and transgenic mice, with an emphasis on conditional alleles, reporter alleles and Cre driver lines.
• Mutant Mouse Regional Resource Centers
• NCI Frederick Cancer Mouse Repository
• NIA Mutant Mouse Aging Resource
• European Mouse Mutant Archive
• Riken BioResource Center, Japan

Conditional Mutant and Cre Driver Archives and Databases
• The Cre-X-Mice database at SLRI allows you to search for Cre-expressing mice by a variety of criteria
• Jax Induced Mutant Resource has an extensive collection of targeted and transgenic mice, with an emphasis on conditional alleles, reporter alleles and Cre driver lines.
• The European Conditional Mouse Mutagenesis Program (EUCOMM) has an ES cell archive with thousands of conditionally targeted or trapped genes accessed with a searchable database

Gene Trapped ES cell Repositories and Databases
• What is a gene trap? Read the Gene Trap Overview provided by the International Gene Trap Consortium
• The International Genetrap Consortium has a searchable database of gene trapped mutant ES cell lines from a variety of centers.
• Texas Institute for Genomic Medicine has a searchable database of gene trapped mutant ES cell lines, including many genes not expressed in ES cell lines. Knockout lines and mice are also available.
• The European Conditional Mouse Mutagenesis Program (EUCOMM) has an ES cell archive with thousands of conditionally targeted or trapped genes accessed with a searchable database
• BayGenomics

Phenotype-driven Screens for Variants
• Phenotypes and Mutants Community Resources at Jax links to many phenotype-driven screens.
• Large Scale Mutagenesis Program, Riken, Japan
• Center for Modeling Human Disease, Canada
• Neuroscience Mutagenesis Facility at Jackson Laboratory
• Mouse Heart, Lung, Blood, and Sleep Disorders Center at Jackson Laboratory
• Harwell ENU Mutagenesis Program, England
• Mutant Mouse Database at Oak Ridge National Laboratory
• Sloan-Kettering Mouse Mutagenesis
• Mouse Mutagenesis Center for Developmental Defects, Baylor College of Medicine

Transgenic Mouse Repository
• Gensat generates BAC transgenic mice to express Cre or EGFP in specific neuronal populations.

Commercial DNA Cloning and Genotyping Services

Vector Construction
• BlueSky Biotech
• GenScript Corporation, knockout vectors starting at $2800
• inGenious
• InvivoGen
• OzGene
• SeqWright
• Vega Biolab

Transgenics
• genOway
• OzGene
• VisGenYX

Gene Targeting
• genOway
• inGenious
• OzGene
• VisGenYX

Genotyping
• Charles River
• Embark Scientific
• MendelWorks
• TransnetYX

Mouse Genetics

• Mouse Genome Informatics at the Jackson Laboratory
• Lee Silver's "Mouse Genetics", published online by the Jackson Laboratory
• "The Coat Colors of Mice" by Willys K. Silvers
• Inbred Strains of Rats and Mice, a reference resource at the Jackson Laboratory
• Origins of Inbred Mice Edited by Robert C Morse III
• emap, the edinburgh mouse atlas project

Rodent Pathology and Medicine

• Pathbase a mouse pathology database at the University of Cambridge
• Mouse Diseases, from the Missouri University College of Veterinary Medicine
• Guidelines for assessing the health and condition of mice by Folz and Ullman Collere, a pdf file of a paper published in Lab Animal. A useful guide to judging the health and welfare of mice from their appearance and behavior.
• Lab Animal Comparative Pathology at UC Davis. A web site resource with lots of useful information about animal pathology.
• The American Committee on Laboratory Animal Diseases (ACLAD) has a web site with useful information on treatment and diagnosis of diseases which affect laboratory animals, and access to an archive of their newsletters.
• Veterinary Formulary, guidance on drug dosages for different species from the University of Minnesota.
• Infectious Diseases of Mice and Rats
• Companion Guide to Infectious Diseases of Mice and Rats
• Microbial Status and Genetic Evaluation of Mice and Rats

Discussion Groups and Listservs

     Listservs are automated email discussion groups. The COMPMED (comparative medicine) listserv is a popular discussion group for vets and IACUC members.

• Vetmed List of Listservs.
• Mouse Genome Informatics listserv.
• transgenic-list, a listserv about creating transgenic and knockout mice.
• COMPMED Comparative Medicine Discussion List. To subscribe, send an e-mail message to listserv@listserv.aalas.org and in the body of the message, enter: subscribe COMPMED Firstname Lastname

Online Books

• Lee Silver's Mouse Genetics, published online by the Jackson Laboratory
• The Coat Colors of Mice by Willys K. Silvers, published online by the Jackson Laboratory
• Origins of Inbred Mice Edited by Robert C Morse III, published online by the Jackson Laboratory
• The Anatomy of the Laboratory Mouse by Margaret J Cook, published online by the Jackson Laboratory
• Biology of the Laboratory Mouse Edited by E.L. Green, published online by the Jackson Laboratory
• Recognition and Alleviation of Pain and Distress in Laboratory Animals
• Monoclonal Antibody Production
• Guide for the Care and Use of Laboratory Animals
• Rodents in Research
• Nutrient Requirements of Laboratory Animals
• Immunodeficient Rodents: A Guide to Their Immunobiology, Husbandry, and Use

Mouse Courses

• Courses at The Jackson Laboratory
• Molecular Embryology of the Mouse, Cold Spring Harbor Laboratory

Academic Targeting and Transgenic Cores Elsewhere

• Rochester
• Ohio State
• UC Irvine
• Penn
• Michigan
• UMass
• MD Anderson
• Cinncinati
• Washington University
• UConn HC
• Tufts