We create transgenic mice, knockout mice (null mutations, knockins, targeted point mutations, humanized genes, conditional knockouts) and chimeric mice, provide rederivation, cryopreservation services and establish embryonic stem cell lines for the Cleveland Biomedical Research Community.

     Services are contracted on a fee-for-service, first-come-first-serve basis. Our rates are low, our wait times are short, and we have an oustanding record of success with many publications resulting from our work.

     We are supported by user fees, the Case Western Reserve University School of Medicine and the Case Comprehensive Cancer Center.

Personnel:
Weihong JiangStaff216 368-2528wxj13@case.edu
David LePageStaff216 368-2528dfl@case.edu
Rachel MannManager216 368-2528rachel.mann@case.edu
Ron ConlonDirector216 368-1826rac14@case.edu



Wait Times:
ServiceTime to Job Start* ( Current as of 11/22/09)
Transgenicsimmediately
Chimerasimmediately
Rederivationimmediately
Cryopreservationimmediately
Gene Targeting1 month
*after receipt and validation of materials and information

 

   Services: Information:
Before You Place An Order:
  • Transgenics and Chimeras: You must have an IBC protocol from CWRU and an IACUC protocol from your institution
  • Rederivations, FET, IVF/ICSI: You must have an IACUC protocol from your institution




     The Case Transgenic and Targeting Facility office is located in Wolstein Research Bldg 2514.

Left to Right: Ron, Rachel, David, Weihong

 

Publications in 2009 of Transgenic and Knockout Mice We Made:
  1. Alexander GM, Rogan SC, Abbas AI, Armbruster BN, Pei Y, Allen JA, Nonneman RJ, Hartmann J, Moy SS, Nicolelis MA, McNamara JO and Roth BL. Remote Control of Neuronal Activity in Transgenic Mice Expressing Evolved G Protein-Coupled Receptors. Neuron 2009 63(1):27-39

    2. • The Roth lab developed transgenic mice in which activity of specific neuronal populations can be increased by a peripherally administered ligand. This approach is a powerful new tool to study mammalian brain function.

  2. Kawanami A, Matsushita T, Chan YY, Murakami S. Mice expressing GFP and CreER in osteochondro progenitor cells in the periosteum. Biochem Biophys Res Commun. 2009

  3. Soler DC, Kadunganattil S, Ramdas S, Myers K, Roca J, Slaughter T, Pilder SH, and Vijayaraghavan S Expression of Transgenic PPP1CC2 in the Testis of Ppp1cc-Null Mice Rescues Spermatid Viability and Spermiation but Does Not Restore Normal Sperm Tail Ultrastructure, Sperm Motility, or Fertility Biol Reprod 2009

  4. Mailankot M, Staniszewska MM, Butler H, Caprara MH, Howell S, Wang B, Doller C, Reneker LW, Nagaraj RH. Indoleamine 2,3-dioxygenase overexpression causes kynurenine-modification of proteins, fiber cell apoptosis and cataract formation in the mouse lens. Lab Invest. 2009 89(5):498-512

  5. Heaney JD, Michelson MV, Youngren KK, Lam MY, Nadeau JH. Deletion of eIF2beta suppresses testicular cancer incidence and causes recessive lethality in agouti-yellow mice. Hum Mol Genet. 2009 18(8):1395-404

  6. Cheng L, Pilder S, Nairn AC, Ramdas S, Vijayaraghavan S. PP1gamma2 and PPP1R11 are parts of a multimeric complex in developing testicular germ cells in which their steady state levels are reciprocally related. PLoS One. 2009;4(3):e4861

  7. Li H, Wang D, Singh LS, Berk M, Tan H, Zhao Z, Steinmetz R, Kirmani K, Wei G, Xu Y. Abnormalities in osteoclastogenesis and decreased tumorigenesis in mice deficient for ovarian cancer G protein-coupled receptor 1. PLoS One 2009 4(5):e5705.

  8. Walker MP, Tian L, Matera AG. PLoS One. Reduced viability, fertility and fecundity in mice lacking the cajal body marker protein, coilin. 2009 4(7):e6171.

  9. Matsushita T, Wilcox WR, Chan YY, Kawanami A, Bükülmez H, Balmes G, Krejci P, Mekikian PB, Otani K, Yamaura I, Warman ML, Givol D, Murakami S. FGFR3 promotes synchondrosis closure and fusion of ossification centers through the MAPK pathway. Hum Mol Genet. 2009 18(2):227-240

  10. Mack JA, Maytin EV. Persistent Inflammation and Angiogenesis during Wound Healing in K14-Directed Hoxb13 Transgenic Mice. J Invest Dermatol. 2009

  11. Zhang T, Yong SL, Drinko JK, Popovic ZB, Shryock JC, Belardinelli L, Wang QK. LQTS mutation N1325S in cardiac sodium channel gene SCN5A causes cardiomyocyte apoptosis, cardiac fibrosis and contractile dysfunction in mice. Int J Cardiol. 2009

  12. Ghosal K, Vogt DL, Liang M, Shen Y, Lamb BT, Pimplikar SW. Alzheimer's disease-like pathological features in transgenic mice expressing the APP intracellular domain. Proc Natl Acad Sci U S A. 2009

  13. Vogt DL, Thomas D, Galvan V, Bredesen DE, Lamb BT, Pimplikar SW. Abnormal neuronal networks and seizure susceptibility in mice overexpressing the APP intracellular domain. Neurobiol Aging. 2009

  14. Qing Li, Danping Huang, Kristine Nacion, Hong Bu and Feng Lin Augmenting DAF levels in vivo ameliorates experimental autoimmune encephalomyelitis Mol Immunol. 2009 46(15):2885-91.

  15. Takehiko Matsushita, Yuk Yu Chan, Aya Kawanami, Gener Balmes, Gary E. Landreth, and Shunichi Murakami ERK1 and ERK2 play essential roles in osteoblast differentiation and in supporting osteoclastogenesis Mol Cell Biol. 2009 29(21):5843-57

  16. Bliss SP; Miller A; Navratil AM; Xie J; McDonough SP; Fisher PJ; Landreth GE; Roberson MS, ERK signaling in the pituitary is required for female but not male fertility., Mol Endocrinol 2009 Jul;23(7):1092-101

  17. Nakamura Y, Cui Y, Fernando C, Kutz WE, Warman ML. Normal growth and development in mice over-expressing the CCN family member WISP3. J Cell Commun Signal. 2009 3(2):105-13

   Publications from previous years




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